The infrastructure of the Omics Research Center

Infrastructure

The infrastructure of the Omics Research Center enables comprehensive analyses in the fields of metabolomics/lipidomics, proteomics/peptidomics, transcriptomics, and genomics.
The equipment includes state-of-the-art scientific instrumentation, including:
LC/MS/MS (triple quadrupole)

LC/MS/MS (triple quadrupole) – used for quantitative metabolomic, lipidomic, and peptidomic analyses

▪ Waters Xevo TQ Absolute UPLC/MS/MS

LC/MS/MS - high-resolution mass spectrometers for qualitative analysis

• LC/MS/MS - high-resolution mass spectrometers for qualitative analysis of the quadrupole time-of-flight (QTOF) type

▪ Waters CYCLIC IMS nUPLC/UPLC/MS/MS high-resolution mass spectrometer equipped with a cyclic ion mobility spectrometry (IMS) module – proteomics, structural proteomics, protein post-translational modifications, peptidomics,
▪ Waters SYNAPT XS nUPLC/UPLC/MS/MS high-resolution mass spectrometer equipped with an ion mobility spectrometry (IMS) module – metabolomics, lipidomics, peptidomics, proteomics, tissue mapping using DESI techniques,
▪ Waters Xevo G2 nUPLC /UPLC/MS/MS - metabolomics, lipidomics, peptidomics

GC/MS/MS (triple quadrupole)
▪ Waters Xevo TQ-GC GC-MS/MS - used for the quantitative analysis of volatile compounds, with liquid injection as well as analysis of compounds with very low boiling points using a headspace sampler
Genome sequencing instruments
▪ Illumina NextSeq 2000 Sequencing System - a high-throughput sequencer enabling a wide range of NGS applications, including exome and transcriptome sequencing in a single run
▪ Illumina MiSeq System - a lower-throughput sequencer designed primarily for gene panel sequencing
Multiplex biomolecule analysis system
▪ Bio-Plex 200 System for multiplex biomolecule analysis using suspension array technology with an automated magnetic–vacuum washer. Enables quantitative analysis of proteins and nucleic acids
Automated microwave peptide synthesizer
▪ Biotage Initiator+ Alstra - Microwave Peptide Synthesizer- capable of synthesizing peptides, miniproteins, and protein subunits of up to 60–70 amino acid residues
In addition, the Center is equipped with numerous auxiliary devices, including: a Bio-Rad CFX96 RT-PCR system; a Labconco FreeZone lyophilization system with a 12-port stainless steel drying chamber; a Heto vacuum concentrator; a Hetovac VR-1 with a cooling system; a µSPE (micro Solid Phase Extraction) manifold for 96-well plates from Waters; an Eppendorf Centrifuge 5430 R; and an Eppendorf ThermoMixer C orbital shaker.